Among the various vaccine against Actinobacillus pleuropneumoniae, subunit vaccine uses a recombinant protein from ApxI, ApxII, and ApxIII as vaccine antigens have shown good success in terms of security and protection. Therefore, subunit vaccines are being implemented around the world and the development of new subunit vaccines is actively being conducted. To evaluate the effectiveness of a subunit vaccine, it is important to measure the immune response to each toxin Apx separately. However, the cross-reactivity of antibodies makes it difficult to measure the immune reactivity specific to each toxin. In this study, region-specific antigen between toxins identified and cloned to solve this problem. Respectively antigenicity of recombinant proteins Hapten Conjugates Proteins demonstrated by Western blot. Using recombinant proteins, we developed Immunosorbent Assay (ELISA) enzyme-linked method that can detect immune response specific for each Apx toxins in laboratory guinea pigs. ...
Despite efforts to control influenza virus infection and transmission of influenza virus still causes significant morbidity and mortality in the global human population each year. Most of the vaccines currently targeting the immunodominant viral surface glycoprotein hemagglutinin. Cat Recombinant Proteins However, reducing the severity of disease and viral shedding has also been associated with antibody targeting the viral surface glycoprotein, the neuraminidase. Importantly, immune antineuraminidase proved to be relatively wide, in contrast to the vaccine-induced antibodies to hemagglutinin head domain. In this study, we assessed the recombinant neuraminidase protein vaccination due to its ability to prevent or limit virus transmission. We vaccinated guinea pigs either intramuscular or intranasal influenza B virus neuraminidase to assess whether vaccination recombinant neuraminidase through these routes can prevent transmission of the virus homologous to the naive recipient. gu...