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Construction, Expression and Evaluation of Recombinant VP2 Protein for serotype-independent Detection of FMDV Seropositive Animals in Egypt.

foot-and-mouth disease virus (FMDV) is one of the most devastating viral pathogens cloven-hoofed animals. Detecting antibody (Ab) against FMDV structural proteins (SP) test virus neutralization (VNT) and liquid phase blocking ELISA (LPBE) is a standard procedure Swine Recombinant Proteins that is used to monitor seroconversion in animals post-vaccination, the prevalence of infection-surveillance, proving the clinical cases and status seronegative of FMDV free / naïve animals before transport. 

However, due to variations in the SP of FMDV serotypes, each serotype-specific Ab must be detected separately exhausting and time-consuming. Thus, it is very important to develop the test is sensitive, rapid, accurate and capable of detecting specific FMDV Ab, irrespective of its serotype. This study describes the VP2 protein heterologous expression in E. coli, and evaluation as an indirect antigen capture in simple ELISA for the detection of serotype-independent anti-FMDV Ab. sequence analysis revealed that the VP2-coding sequences conserved between FMDV serotype far. 

The recombinant VP2 (rVP2), 22 kDa polypeptide, purified to near homogeneity by affinity chromatography under native conditions. Immunoreactivity of rVP2 confirmed by using a panel of positive sera included sera from animals vaccinated with trivalent vaccine antiserum and guinea pig local FMDV, which are routinely used as tracing / Ab detected in testing LPBE. The results of the ELISA-based VP2 comparable to those determined by VNT and LPBE standard diagnostic tests. RVP2 specificity and sensitivity in capturing the anti-FMDV Ab was 98.3% and 100%, respectively. 
Construction, Expression and Evaluation of Recombinant VP2 Protein for serotype-independent Detection of FMDV Seropositive Animals in Egypt.

The forward VP2-ELISA assay proved to be a reliable and time saving for detecting FMDV seropositive animals, regardless of FMDV serotype Bovine Recombinant Proteins that can be implemented in combination with VNT and / or LPBE for rapid diagnosis of FMDV infection is ongoing.
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